Skip to main content
. Author manuscript; available in PMC: 2013 Aug 28.
Published in final edited form as: Free Radic Biol Med. 2011 Jul 4;51(8):1533–1542. doi: 10.1016/j.freeradbiomed.2011.06.028

Fig. 5. Knock down of TR1 does not alter cytoplasmic activation or nuclear DNA binding of NFκB.

Fig. 5

HeLa cells were transfected with siTR1 or transfection controls consisting of non-targeting siRNA (siNT), mock transfection (mock; no siRNA) or untransfected (unt; no siRNA or Dharmafect transfection reagent) for 48 hours, then stimulated with 10 ng/ml TNF for 1 hour where indicated. (A) Western blot of cytoplasmic IκB-α. (B) Western blot of nuclear proteins for p50 and p65 levels. Blots were re-probed for β-actin to demonstrate equal protein loading. (C) Nuclear proteins were analyzed by EMSA as described in the legend to Fig. 3.