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. Author manuscript; available in PMC: 2014 Jul 1.
Published in final edited form as: J Immunol. 2013 May 29;191(1):472–479. doi: 10.4049/jimmunol.1300521

Figure 2. TCRα LCR-dependent hCD2 reporter protein expression in T-cells differentiated from ES cell clones.

Figure 2

(A) Flow cytometry analyses of hCD2 expression in three representative independent clones of ESC transfected with hCD2ΔT (ΔT) and subsequently differentiated into CD4,CD8 double positive (DP) and CD8 single positive (SP) T-cells. None of the clones produce T cells that are hCD2 positive (n=6). (B) DP and CD8 SP T cells derived from three representative independent ESC clones transfected with hCD2:1-8 are positive for hCD2 reporter protein. All hCD2:1-8 ESC clones (1-8:) produce T cells that express the hCD2 protein on their cell surface (n>12). Cell population gates are shown at left. hCD2 expression in transfected (dark curve) and non-transfected (light curve) gated cells is shown at right.