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. Author manuscript; available in PMC: 2014 Jul 1.
Published in final edited form as: J Immunol. 2013 May 29;191(1):472–479. doi: 10.4049/jimmunol.1300521

Figure 4. Appropriate upregulation of the hCD2:1-8 reporter gene at DN3 or during the DN3 to DP stage transition of in vitro T cell development.

Figure 4

Flow cytometry analysis of T cell development from four representative hCD2:1-8ESC clones (1-8:) differentiated in the OP9DL1 co-culture system. Cells were harvested on day 12 of co-culture to detect CD4, CD8 double negative (DN)1 (CD44+CD25neg) and DN2 (CD44+CD25+) stage T cells, or day 18 to examine DN3 (CD44negCD25+) and DP (CD4+CD8+) cells. Note that clone 1-8:A1 also appears in Figure 3. Representative target cell population gates are shown at top. hCD2 expression in gated transfected (dark curve) and non-transfected (light curve) cells is shown below in each column.