. Author manuscript; available in PMC: 2013 Aug 28.

Published in final edited form as: Biometals. 2007 Dec 6;21(3):353–366. doi: 10.1007/s10534-007-9124-5

Table 5.

Predicted residues incorporated by NRPS adenylation domains of P. mendocina ymp

ORF	Module	10 amino acid code^a	Predicted amino acid^a	NRPS gene containing a similar amino acid code (residue incorporated/10 amino acid code)^b,^c
Pmen1902	1	DMMSASSLY	No match	pvdL(Acyl-cap/DMGSDSCLTK)
Pmen1902	2	DLTKIGHVGK	Asp
Pmen1902	3	DIWELTADDK	No match	pvdL(Dab/DIWELTADK)
Pmen1901	1	DVWHVSLIDK	Ser
Pmen1901	2	DGEACAGVTK	No match	pvdJ(fOHOrn/DGEVCGGVTK)
Pmen1901	3	DVWHVSLIDK	Ser
Pmen1901	4	DGEACAGVTK	No match	pvdJ(fOHOrn/DGEVCGGVTK)

Amino acid codes were identified via NRPS gene alignments made by NRPSpredictor Program. Predictions of the encoded amino acid were generated from the same program (see text)

When an encoded amino acid could not be predicted using this program, a manual method was applied. Amino-acid-encoding regions from several Pseudomonad-derived NRPSs producing structurally characterized siderophores were identified using the same program (from P. aeruginosa PAO1, P. aeruginosa ATCC27853, P. aeruginosa Pa6, P. fluorescens 9AW, P. putida CFB2461; see Thupvong et al. (1999). These were compared manually with the coding regions identified for the pair of P. mendocina NRPSs. Best matches are shown

Dab:2,4-diaminobutyric acid, fOHOrn: N⁵-hydroxy-N⁵-formyl-L-ornithine