Fig. 1.
Comparison of virion neutralization by gH–gL-specific mAbs T2C12 and 8F10. MuHV-4 virions expressing eGFP from an intergenic EF1α promoter were incubated (2 h, 37 °C) with antibody dilutions, then divided between RAW-264 monocytes (3 p.f.u. per cell), BHK-21 fibroblasts (0.5 p.f.u. per cell) and NMuMG epithelial cells (0.5 p.f.u. per cell). After overnight incubation (37 °C) in the presence of 100 µg phosphonoacetic acid ml−1 to prevent secondary spread, the cells were analysed for eGFP expression by flow cytometry. The MHC class I-specific IgG2a mAb 28.14.8 provided a control.