Fig. 2.

Immunofluorescence analysis of ABCD1 expression in normal, X-ALD, and CADDS fibroblasts. Expression of mutant ABCD1 proteins in fibroblasts was analyzed by immunofluorescence analysis. Normal, X-ALD, and CADDS fibroblasts were stained with anti-ABCD3 and anti-ABCD1 antibodies followed by secondary antibodies conjugated with the dye reagents. Fluorescent dots were observed under confocal microscopy. Bar = 20 μm. ABCD1 protein was not detected in X-ALD and CADDS fibroblasts