Fig. 3.

VLCFA β-oxidation activity and ABCD1 protein expression in X-ALD fibroblasts. VLCFA β-oxidation activity in the normal, ALD, βand CADDS fibroblasts was measured using [1-¹⁴C]C24:0 as substrate (a). Results are the means ± S.D.; n = 3. Expression of ABCD1 proteins in each of the cells was analyzed by immunoblot analysis (b). The C24:0 β-oxidation was less than 20% of the normal fibroblasts and the ABCD1 protein was not detected in the X-ALD fibroblasts