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. Author manuscript; available in PMC: 2013 Aug 28.
Published in final edited form as: Brain Cell Biol. 2008 Apr 5;35(0):267–281. doi: 10.1007/s11068-008-9018-7

Fig. 3.

Fig. 3

The putative complex is exceptionally stable.

A. Western blot of nuclear and cytosolic fractions from rat forebrain, probed with anti-pERK1/2 antibody. Samples in SDS-PAGE sample buffer were boiled for 5, 15, or 30 minutes.

B. Western blot of nuclear and cytosolic fractions from rat forebrain, probed with anti-ERK1/2 antibody. Samples in SDS-PAGE sample buffer were boiled for 5, 15, or 30 minutes. Similar findings were observed when samples were boiled with 6 M GdnHCl, 9 M urea, or 10% SDS.