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. Author manuscript; available in PMC: 2014 Jul 25.
Published in final edited form as: Immunity. 2013 Jul 18;39(1):97–110. doi: 10.1016/j.immuni.2013.06.012

Figure 2. FcεR1α+ GMPs Possess the Ability to Express IL-4, IL-6, and IL-13.

Figure 2

(A) The gates for sorting FcεR1α− and FcεR1α+ GMPs. The sorted FcεR1α− and FcεR1α+ GMPs were stained with May-Grunwald Giemsa. (B) The sorted cells were stimulated with P&I for 4 hours. mRNA expression was measured by qPCR (mean ± SD, triplicates). Ba=basophils; MC=BMMC. (C) The sorted FcεR1α− and FcεR1α+ GMPs were not cross-linked (NCL) with IgE, cross-linked with IgE (IgECL) or stimulated with P&I for 4 hours. (D) The sorted cells were stimulated with P&I overnight. IL-4, IL-6, and IL-13 proteins were measured by ELISA (mean ± SD, triplicates). Data represent two independent experiments with similar results.