Figure 1.
HDAC3 suppresses hepatic lipogenesis. (A) Oil Red O staining of neutral lipid in liver from 12-week-old HDAC3f/f mice 2weeks after intravenous injection of adenovirus-associated virus (AAV) expressing either green fluorescence protein (GFP) or Cre recombinase under a hepatocyte-specific thyroxin-binding globulin (TBG) promoter. (B) Hepatic triglyceride (TG) levels were measured by enzymatic colorimetric assay. Error bars indicate S.E.M. * P < 0.05. (C) Hepatic de novo lipogenesis (DNL) rate was measured in 12-week-old HDAC3f/f mice 1 week after injection with AAV-Cre in the light cycle, by tracing synthesis of 2H-labeled hepatic palmitate from 2H2O injected intraperitoneally. Error bars indicate S.E.M. * P < 0.05. (D) Transcription of genes (in red) involved in fatty acid synthesis was upregulated in the light cycle in liver from HDAC3f/f mice at 1 week after AAV-cre injection. Cs, citrate synthase; Acacb, acetyl-CoA carboxylase 2; Fasn, fatty acid synthase; Elovl, fatty acid elongase; Scd1, stearoyl-CoA desaturase 1; Gpam, glycerol-3-phosphate acyltransferase 1, mitochondrial; Acss, acyl-CoA synthetase; CD36, fatty acid translocase.