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. Author manuscript; available in PMC: 2013 Aug 28.
Published in final edited form as: Cold Spring Harb Symp Quant Biol. 2011 Sep 6;76:49–55. doi: 10.1101/sqb.2011.76.011494

Figure 2.

Figure 2

Chromatin occupancy of HDAC3 is circadian in anti-phase to hepatic lipogenesis. (A) Hepatic lipogenesis and expression of lipogenic genes is circadian with a zenith in the night cycle and a nadir in the light cycle in nocturnal animals such as mouse and rat. ZT: Zeitgeber time that marks the light/dark cycle -- light on at ZT0, light off at ZT12. (B) Circadian rhythm of hepatic HDAC3 chromatin occupancy on lipogenic genes is inversely associated with rhythmic acetylation at lysine 9 on histone 3 (acH3K9) and RNA polymerase II (Pol II) recruitment. Deletion of HDAC3 causes constitutive histone acetylation. Occupancy of HDAC3, acH3K9, and Pol II on the genome was determined by ChIP-seq and viewed by the UCSC genome browser. The y-axis indicates relative signal intensities that were normalized to reads per million (rpm) and put into the same scale between ZT10 and ZT22 for the same factor for easy comparison. Gpam, glycerol-3-phosphate acyltransferase 1, mitochondrial; Elovl5, fatty acid elongase 5; Scd1, stearoyl-CoA desaturase 1.