Skip to main content
. Author manuscript; available in PMC: 2014 Sep 1.
Published in final edited form as: Biomaterials. 2013 Jun 15;34(28):6773–6784. doi: 10.1016/j.biomaterials.2013.05.061

Fig. 2.

Fig. 2

SIMS mouse submandibular cell line conforms to the shape of 30 μm radius nanofibrous craters after 96 h of growth. SEM images show cell conformity from (A) top-down and (B) angled views. (C) A tilted view of a fluorescence confocal Z-stack 3-D projection with SIMS stained for F-actin (green, phalloidin), and nuclei (blue, DAPI), conforming to a crater lined with nanofibers (red). The Z-plane is denoted by the arrow. The arrangement of nuclei on (D) 30 μm, (E) 80 μm radius craters, and (F) flat nanofibers, reveals that nuclei preferentially localize within the craters, more obviously on 30 μm radius craters. Dotted circles outline a portion of the craters in each sample. On 80 μm radius craters, some regions exhibited cell overlapping (red arrows), whereas on 30 μm radius craters and flat nanofibers, cells grew largely as one monolayer over the entirety of the samples.