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. Author manuscript; available in PMC: 2014 Apr 2.
Published in final edited form as: Structure. 2013 Mar 14;21(4):560–571. doi: 10.1016/j.str.2013.02.005

Figure 5. Steady State Localization of Transport Reporter after Depletion of Nup192.

Figure 5

(A) The different panels show the localization of the Nab2NLS-mCherry-PrA NPC function reporter protein in strains where Nup192, Nup145, or Nup82 levels were repressed with chlortetracycline. Example images of these strains are shown after 0 or 24 hr of repression.

(B) The N/C ratios of the reporter were quantified from ~100 cells per condition and normalized to a WT N/C value of 1 at the respective time point. These mean values are plotted with error bars of the standard error of the normalized means. Reported p values were the result of a Mann-Whitney rank sum test, comparing the distributions of normalized N/C values with or without repression. See also Figure S8.