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. Author manuscript; available in PMC: 2013 Aug 28.
Published in final edited form as: J Mol Biochem. 2012 Feb 15;1(1):26–39.

Figure 3.

Figure 3

ELISA analysis was used to validate the binding integrity of the CRAds used in this study. Integrins were bound to a 96-well plate followed by binding of CRAds at the listed dilutions. Anti-Ad mouse antibody followed by goat anti-mouse secondary antibody conjugated to Horse Radish Peroxidase allowed for quantitative measurement of the amount of virus bound to integrins using a light-emitting reaction detectable by excitation at OD405. Both Δ24DoubleRGD and Δ24IXRGD bind αvβ3 integrin significantly better than Δ24 and Δ24FiberRGD (A). Each of the RGD-modified CRAds binds αvβ5 significantly better than Δ24 (B).