Crystal violet analysis of SKOV3.Luc cells following 10-days of infection with CRAds at listed VP/cell indicated that Δ24DoubleRGD causes more CPE, as determined by less staining of remaining cells, as compared to Ad5 non-replicative control vector, Δ24Ad5, Δ24IXRGD, and Δ24FiberRGD (A). Δ24DoubleRGD also caused increased killing of SKOV3.Luc cells as determined by light emitted from cells following administration of MTS reagent and read on plate reader at OD405 (B). Less light emitted is indicative of increased CPE, less living cells, at the time of reading.