Figure 4.

Direct brain injections of scAAV9/cre virus into Mecp2^stop mice result in predominantly neuronal-specific MeCP2 expression and a mild improvement of behavioral phenotypes. A, Schematic showing site of injections (*). B, Experimental paradigm for males. C, Immunostaining of representative striatal section from a Mecp2^stop/y mouse injected with scAAV9/cre shows MeCP2 expression in neurons (NeuN⁺). Scale bar, 200 μm. D–G, Quantification of immunostaining data (n = 3). Data are means ± SEM. Cell counts are relative to total DAPI-positive cells (D), total NeuN-positive cells (E), total NeuN-negative cells (F), and total MeCP2-positive cells (G). H, Experimental paradigm for females. I, Observational scores for female Mecp2^Stop/+ mice injected with scAAV9/cre (n = 5), scAAV9/Control (n = 5), and noninjected Mecp2^+/+ mice (n = 12).