Figure 2.

RSD-induced anxiety was associated with increased recruitment of LysM-GFP⁺ monocytes from circulation into the brain. LysM-GFP⁺ mice were subjected to six cycles of social defeat (RSD) or left undisturbed as controls (CON). Anxiety-like behavior was tested 14 h after RSD. Following behavioral testing, blood and enriched brain CD11b⁺ cells were collected (n = 6–8, 2 independent experiments). A, Time to enter the center of the open field. B, Time spent in the open field. C, Average spleen weight. D, Representative flow bivariate dot plots of SSC/Ly6C on CD11b+ cells. E, The percentage of CD11b⁺/SSClo/Ly6C^hi monocytes in the blood. F, Representative bivariate dot plots of GFP/CD11b labeling of Percoll-isolated microglia/macrophages. G, The percentage of GFP⁺/CD11b⁺ cells in the brain. H, The number of GFP⁺/CD11b⁺ cells that were CD45^hi or CD45^lo. I, Representative histograms of GFP expression in blood monocytes (CD11b⁺/Ly6C^hi) and brain macrophages (CD11b⁺/CD45^hi). J, Relative MFI of GFP⁺/CD11b⁺ cells in the blood or brain. Bars represent the mean ± SEM. Means with asterisk are significantly different from CON (p < 0.05).