Figure 3.

LysM-GFP⁺ macrophages were present in the PVS of the brain following RSD. LysM-GFP⁺ mice were subjected to six cycles of social defeat (RSD) or left undisturbed as controls (CON). Brains were collected 14 h after RSD for histology (n = 4–6, 2 independent experiments). A, B, Representative images of LysM^GFP (green), merged LysM^GFP/Iba-1 (red), and merged LysM^GFP/Iba-1/Ly6C (blue) in the PFC of CON and RSD mice. C, D, Representative images of LysM^GFP (green), merged LysM^GFP/Iba-1 (red), and merged LysM^GFP/Iba-1/Ly6C (blue) immunofluorescence in the HPC of CON and RSD mice. White arrowheads point to perivascular LysM-GFP⁺ macrophages. Scale bars, 100 μm.