FIGURE 4.

CD8 engagement with MHC/ TCR induces altered signaling patterns, as compared with MHC/TCR alone. A, CD8 P14 splenocytes were stimulated for 3 min with tetramers at 37°C, and phosphotyrosine activity was detected by Western blot. Cells were unstimulated (US) or stimulated with D^b/gp33 (lane 1), D^b D227K/gp33 (lane 2), D^b/C9M (lane 3), or D^b D227K/C9M (lane 4). Data are representative of three independent experiments. B, Quantitation of band intensity of tetramer-stimulated cells. To measure the intensity of the bands, pixel volume of identical areas of the gel were determined for each lane and corrected for local area background. Two bands missing in the D^b D227K stimulated and one control band are plotted. C, Calcium mobilization of cells treated with indicated tetramers. Data are representative of two independent experiments.