FIGURE 5.

CD8 is present in mi-crodomains without engagement by MHC. A, CD8⁺ P14 splenocytes were stained with anti-CD8α and D^b/C9M (top), D^b D227K/C9M (middle), or D^b tetramer containing an irrelevant peptide (D^b/flu, bottom). Tetramer staining is shown in red, CD8 staining in green, and colocalization of tetramer and CD8 in yellow. CD8 colocalizes with tetramer in discrete patches (foci, arrows) with and without tetramer engagement of CD8. Data are representative of at least three independent experiments. B, Calculated colocalization of CD8 and tetramer.