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. Author manuscript; available in PMC: 2013 Aug 28.
Published in final edited form as: Biochem J. 2009 Dec 23;425(2):445–543. doi: 10.1042/BJ20090380

Figure 4. The effect of PRL on the autophosphorylation of PKN and PKCs.

Figure 4

Autophosphorylation activity of human PKN1 or PKC was detected by autoradiography. The concentration of the PRL peptide was 0, 0.5, 1, 5 and 20 μM respectively. The results were plotted as a percentage of kinase activity observed in the absence of the PRL peptide (lower panel). The kinase activity in the absence of the PRL peptide was taken as 100 % activity. The results shown are representative of three independent experiments. Human PKN1, ●; rat PKCβII, ○; rat PKCα, +; rat PKCγ, ◇; rat PKCε, □; mouse PKCλ, −; rat PKCζ, ×.