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. Author manuscript; available in PMC: 2013 Aug 28.
Published in final edited form as: Cancer Res. 2012 Oct 16;72(24):6502–6511. doi: 10.1158/0008-5472.CAN-12-1909

Figure 6.

Figure 6

Ectopic expression of CRTC1 partially reverses the inhibitory function of LKB1 upon colony formation, migration, invasion, and metastasis of lung cancer cells. A and B, representative photos (A) and statistic analyses (B) of colonies formed in soft agar of three different groups of A549 cells as indicated. Scale bar, 100 μm (A). Data are shown as mean ± SEM. **, P < 0.01. C and D, ectopic expression of CRTC1 partially reversed the inhibitory effect of LKB1 upon A549 cell migration ability (C) and cell invasiveness in Matrigel (D). Scale bar, 200 μm (C) and 40 μm (D). E and F, ectopic expression of CRTC1 partially reverses the inhibitory function of LKB1 upon metastasis of A549 cells. Representative photos show the gross metastatic nodules on lung surface (E, indicated by the red arrows) as well as the histology of lung metastasis (F) from nude mice received with A549 cells with ectopic LKB1 and/or CRTC1 expression via tail vein injection (5 mice per group). Metastatic incidence is also shown on the bottom. Scale bars, 500 μm (top) and 100 μm (bottom).