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. Author manuscript; available in PMC: 2013 Aug 28.
Published in final edited form as: Immunol Lett. 2010 Jan 12;129(1):32–38. doi: 10.1016/j.imlet.2009.12.022

Figure 4. Anti-Env antibody titers.

Figure 4

Mice (5 mice per group) were DNA immunized (weeks 0 and 3) with Envgp120 alone or fused to two copies of C3d wild-type or mutants. Sera collected two weeks after the last immunization (week 5) was tested for anti-Env antibody titers by ELISA. Different sera dilutions were assayed and the O.D. reported (A). Envgp120- C3d2 wild-type (white square) enhanced the anti-Env antibody titers compared to Envgp120 alone (black circle). Envgp120- C3d2 (R162A) did not enhance or reduce the adjuvant effect of C3d. Envgp120- C3d2 (D163A) (inverted white triangle) and Envgp120-C3d (N170R) (black rhomboid) reduced the adjuvant effect of C3d. However, these mutations did not completely eliminate it, since higher Ab titers than in the Envgp120 alone group were detected. Introduction of the double mutation (D163A-N170R) in C3d (white circle) resulted on almost complete elimination of the adjuvant effect. (B) The results of a 1:200 dilution are shown. Columns indicate the mean and bars ± SEM. The different groups were compared to Envgp120- C3d2 wild-type for statistical analysis (* p<.01, ** P,0.0001).