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. 2013 Sep 1;3(9):1531–1538. doi: 10.1534/g3.113.006791

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Copyright © 2013 Clemmons and Wasserman

This is an open-access article distributed under the terms of the Creative Commons Attribution Unported License (http://creativecommons.org/licenses/by/3.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Maternal tube expression is dramatically reduced in tub^var/tub^null females. (A) Quantitation of tube mRNA in embryos. Quantitative RT-PCR data of tube expression in embryos from females of the specified genotype. Expression data were normalized to rp49 expression and are presented as a fraction of tub⁺/tub^null expression, with tub⁺/tub^null set to 0.5. Error bars represent S.E.M., ***P < 0.001. (B) Quantitation of Tube protein in embryos. Immunoblot using α-Tube sera of protein isolated from embryos from females of specified genotype. Loading control is α-Diaphanous.