Figure 5.

Additional mutations in the tub^var chromosomes genetically interact with the opus insertion to enhance the variable phenotype. (A) Schematic of locations of identified mutations in tub^var chromosomes, including opus insertion, tub⁶ missense mutation, tub⁷ Stalker2 insertion, and tub⁹ P-element insertion. (B) Phenotypic ranges of embryos from females of specified genotype in trans to tub^null. Precise excisants (tub^9Δ) were generated using a transposase source (Δ2-3). GS7007, GS13951, and CB06923 are chromosomes containing P-element insertions within 4 bp of the tub⁹ P-element insertion site. (C) Quantitation of tube mRNA in embryos from females bearing P elements downstream of tube. Quantitative RT-PCR expression data were first normalized to rp49 expression and are presented as a fraction of tub⁺/tub^null expression, with tub⁺/tub^null set to 0.5. Error bars represent SEM. ***P < 0.001, n.s. = not significant.