Figure 1.

Maternal loss of miR-969 and miR-9c leads to reduced primordial germs cell (PGC) numbers. (A) A wild-type embryo at developmental stage S10. PGCs are labeled with α-Vasa (yellow). At S10, PGCs are spread out dorsally toward the embryo surface. (B) Average PGCs numbers in two different laboratory control strains (± SD). (C) Average PGC numbers in embryos derived from miR-969 mutant mothers are reduced in comparison with control and rescued embryos. Error bars correspond to the SD (± SD). Genotypes are (a) Df(1)BSC352/miR-969^KI;;T-969@Fb/+, (b) Df(1)BSC352/miR-969^KI, and (c) Df(1)BSC352/+. (D) Average PGC numbers in embryos derived from miR-9c mutant mothers are reduced in comparison with control and rescued embryos. Genotypes are (d) miR-9c^KO/miR-9c^KO;T-9c@Fb/+, (e) miR-9c^KO/miR-9c^KO;+/nosGal4, (f) miR-9c^KO/miR-9c^KO;T-9c@Fb/nosGal4, (g) miR-9c^KO/+;T-9c@Fb/+, and (h) +/miR-9c^KO;+/nosGal4