Skip to main content
. 2013 Aug 28;8(8):e72920. doi: 10.1371/journal.pone.0072920

Figure 1. RpoN is involved in H. pylori 26695 survival during the stationary phase.

Figure 1

(A) RT-PCR analysis of the mRNA levels of sigma factors in H. pylori 26695 at different growth stages. Data are mean ± SEM from 3 independent experiments. (B) The survival of ΔrpoN strain was deficient during the stationary phase. Bacteria were cultured in Brucella broth containing 10% fetal bovine serum, and numbers of colony formation units (CFU) per milliliter were determined at the times indicated. A representative assay of at least 3 trials is shown. Data are mean ± SEM from 3 independent experiments. (C) Lack of nutrition induces coccoid transformation and death of H. pylori cells. Wild-type and ΔrpoN mutant strain were cultured in liquid medium for 144 h. Cells stained with membrane-permeant SYTO 9 (green) and membrane-impermeant PI (red) were visualized by confocal microscopy. Data are representative of 3 independent experiments.