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IgG isotypes and IgA antibody levels were measured at 30, 60, 180 day post-challenge in all the three groups. (A) High levels of IgG1, (B) IgG2a, (C) IgG2b and (D) IgA antibodies were found at all time points. Bars represent the mean ± S.E (n = 13). The statistical significance based on the differences between the mean values of immunized and control groups are indicated as *p<0.05; **p<0.01 and ***p<0.001.
