Figure 5. Immunization resulted in to significant reduction in parasite burden.

(A) Microfilaraemia in the tail blood of immunized and control groups of M. Coucha were determined after 90 days post challenge. Significant reduction in microfilarial density was observed in Bm-TPP immunized M. coucha throughout the observation when compared to PBS and adjuvant controls. (B) Sterility of female worms was determined by observing the intrauterine contents of female on day 180 post challenge. The female bearing degenerated eggs, embryo, mf were considered sterile. Vaccination with Bm-TPP induces significant sterilization of female worms. Statistically significant percentage of females recovered from immunized mastomys was found sterilized. One dot represents the percentage of female worms recovered from one animal. Bars represent the mean ± S.E. (n = 13) and the statistical significance based on the differences between the mean values of immunized and control groups are indicated as *p<0.05; **p<0.01 and ***p<0.001.