Figure 1. Non-denaturing gradient gel of fluorescently-labeled apoA-I added to human plasma.

ApoA-I labeled with Alexa350 was incubated at 37°C at increasing concentrations with plasma from a healthy human donor and examined by polyacrylamide gel electrophoresis on a 4–20% Tris-glycine gel. Lanes 1 and 2 contain reference samples of purified LDL and HDL, respectively. Fluorescence imaging was performed prior to staining with Coomassie (excitation light, 365 nm; emission blue filter 440–480 nm). The figure is a composite of fluorescence and protein stain images of the same gel.