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. 2013 Aug 28;8(8):e71758. doi: 10.1371/journal.pone.0071758

Figure 3. Functional analyses of Nef clones from treatment and placebo arms.

Figure 3

Panel A: Selected Western blot results depict control SF2 Nef, empty (delta-Nef) plasmid and four samples each obtained from participants in the 1% tenofovir gel and placebo study arms. Panel B: Results for the CD4 downregulation activity of participant-derived nef isolates are shown, normalized to control SF2 Nef (which is equal to 1.0). Panel C: Results for the HLA-A*02 downregulation activity of participant-derived nef isolates are shown, normalized to control SF2 Nef (equal to 1.0). No significant differences in Nef-mediated CD4 or HLA downregulation function were observed between study arms (Mann-Whitney, p = 0.2 and p = 0.06, respectively).