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. 2013 Apr 16;2(3):148–159. doi: 10.4161/adip.24589

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Copyright © 2013 Landes Bioscience

This is an open-access article licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported License. The article may be redistributed, reproduced, and reused for non-commercial purposes, provided the original source is properly cited.

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graphic file with name adip-2-148-g4.jpg — Figure 4. Adipogenesis of Wagyu-IMF derived DFAT cells. (A) Lipid-laden adipocyte derived DFAT cells possessed fibroblast-like appearance with undetectable lipids. (B) 10 d after DMI inducement, 30% DFAT cells of the 100% confluence group accumulated obvious lipid droplets inside cytoplasm which could be stained by ORO staining (C); while DFAT cells of the 80% confluence group showed less than 10% redifferentiation efficiency (D) which was confirmed by ORO staining (E). (F) 10 d after the basal medium culture, DFAT cells of the 100% confluence group showed numerous ORO stain-free vesicles (black arrows). (A, B, D, and F) 200× magnification; (C and E) 400× magnification.