Figure 4. Comparison of adipocyte size distributions created using manual and automatic methods. Comparison between chow (CH) and high-fat (HF) fed adipocyte size is shown for manual (A) and automated methods (B). Cell size distributions in depots from CH animals were very similar when either manual or automated methods were used (C). Similar results were also seen for measurements from HF-diet fed animals, although an underestimation of the smallest groups (<500 µm²) was observed with manual counting, as was a slight rightward shift in the manual curve, consistent with size over-estimation (D).