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. Author manuscript; available in PMC: 2013 Aug 29.
Published in final edited form as: Curr Opin Rheumatol. 2012 Nov;24(6):642–648. doi: 10.1097/BOR.0b013e328356d9dc

Figure 3. Hypermigration toward CXCL12 by healthy AA and SSc monocytes.

Figure 3

Monocyte migration was quantified using microchemotaxis chambers (Neuroprobe) with 100 ng/ml CXCL12 in the lower wells. Human monocytes (50 μl at 1 × 106 cells/ml) were placed in the upper wells in medium supplemented with 5 μM CSD or control peptide. After 3 h at 37°, the filters separating the upper and lower wells were removed, fixed, and stained with DAPI. Cells that had migrated to the lower side of the filter were counted in six high power fields per filter. The data presented are the average ± s.e.m. of experiments performed using cells from 10 healthy Caucasians, 10 healthy AA, and 10 SSc patients. Compared to Caucasian, in the absence of CSD p < 0.0001 for both AA and SSc.