Figure 4. Molecular cloning of GPS.

(a, b) High-resolution linkage map of GPS region produced with 8308 F₂ plants in the Koshihikari background (a) and 2784 F₂ plants in the Takanari background (b). Populations were produced by crossing each cultivar with the corresponding NIL-GPS. The number of recombinants between molecular markers is indicated below the second line in each figure part. Yellow-green shading, regions homozygous for alleles from Koshihikari; dark green, Takanari. Each purple bar in the photosynthesis rate graphs represents the mean ± s.d. (n = 6) of the adjacent genotype. ***P < 0.001; **P < 0.01; n.s., not significant within pairs of recombinant lines (Student's t-test). (c) Gene structure and mutation sites of NAL1 in Koshihikari, Takanari, and nal1 mutant line in Taichung 65 genetic background (T65-nal1). Light blue bars represent exons; white bars represent 5′ and 3′ untranslated regions.