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. Author manuscript; available in PMC: 2013 Aug 29.
Published in final edited form as: Clin Cancer Res. 2012 May 22;18(11):3030–3041. doi: 10.1158/1078-0432.CCR-11-3091

Figure 2.

Figure 2

Parental U251 (WT=wild type) cells were infected with a control or MARCKS shRNA knockdown (MARCKS) lentiviral vector and selected for antibiotic resistance as described in Methods. The control and knockdown cell lines were probed for MARCKS, phosphorylated Akt on serine 473 (pAkt Ser473), total Akt, and GAPDH (for protein normalization) by immunoblotting (A). Proliferation was assessed using ATPlite Luminescence Assay System with mean and standard error shown (** = p<0.01) (B). Clonogenic survival assay was performed on U251 cells infected with either control or MARCKS shRNA (Knockdown) lentiviral vector and plotted in a semi-logarithmic fashion as mean surviving fraction (with standard error of the mean) vs. radiation dose in Gy (C). An upward and rightward shift indicates radiation protection.