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. Author manuscript; available in PMC: 2014 Jun 7.
Published in final edited form as: Circ Res. 2013 Apr 10;112(12):1557–1566. doi: 10.1161/CIRCRESAHA.112.300658

Figure 1. miR-17-92 is required for cardiomyocyte proliferation in embryonic, postnatal and adult hearts.

Figure 1

(A)Haematoxylin and Eosin (H&E) staining of sagittal sections of hearts from 2 days old wild type, heterozygote and mutant miR-17-92-KO mice. Bar = 1 mm.(B) Immunohistochemistry of phosphorylated histone H3 (phospho-H3, pH3) on sagittal sections of hearts from 2 days old wild type, heterozygote and mutant miR-17-92-KO mice. Bar = 100 μm. (C) Immunohistochemistry of sagittal sections of hearts from 2 days old wild type, heterozygote and mutant miR-17-92-KO mice. White boxes are enlarged in lower panels. pH3 labels proliferating cells; α-actinin (ACTN1) marks cardiomyocytes; DAPI labels nuclei. Bars = 50 μm in upper panel and 20 μm in lower panel. (D) Quantification of pH3 positive cardiomyocytes in wild type, heterozygote and mutant miR-17-92-KO hearts from 2 days old mice. **P<0.01 between genetic groups. (N=4~5 each group). (E)Heart weight (HW) to body weight (BW) ratios of 1 year old wild type, heterozygote and mutant miR-17-92-KO mice. N of each genotype group listed. (F)3D reconstruction from confocal images showing intact isolated adult mouse cardiomyocytes. Pan-cadherin stains desmonsomes to demonstrate intact cardiomyocytes (green). α-actinin marks intact sarcomeres (red). DAPI labels intact nuclei. 1 unit = 42.45 μm. (G)Morphology of freshly isolated adult cardiomyocytes from hearts of miR-17-92-KO and control mice. ACTN1 marks rod shaped cardiomyocytes (lower panel). Bars = 250 μm. (H)Quantification of total isolated adult cardiomyocytes from hearts of miR-17-92-KO and control mice. N of each genotype group is listed.(I)Quantitative measurement of the size of freshly isolated adult cardiomyocytes from hearts of miR-17-92-KO and control mice. One hundred individual cells from three different hearts were analyzed per group. ** P< 0.01 between genetic groups.