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. Author manuscript; available in PMC: 2014 Sep 1.
Published in final edited form as: J Immunol. 2013 Jul 31;191(5):2474–2485. doi: 10.4049/jimmunol.1202429

FIGURE 5.

FIGURE 5

Exposure to PR8 elicits a rapid and robust memory CD4+ T-cell response following pH1N1 infection in both lean and obese mice. BAL cells from mice challenged with a secondary heterologous pH1N1 infection were stimulated for 6h with heat-inactivated pH1N1 (n=5–6), pH1N1 pulsed APCs (n=4–6) or Con A (n=5–6). A, Representative flow cytometry gating scheme at 5 dpi, including mean % (SEM). B and C, Number of IFN-γ (B) and GzB (C) producing CD4+ T cells after stimulation with pH1N1 pulsed APCs, n=4–6. D and E, Number of IFN-γ (D) and GzB (E) producing effector memory CD4+ T cells after stimulation with pH1N1 pulsed APCs at 5 dpi, n=4–6. Each bar represents the mean ± SEM.