Table 1. Summary of Inhibition Values.
inhibitor | Tracer | IC50 or Ki (mM) | |
---|---|---|---|
PfENT4 | adenine | [2,8-3H]adenine | 0.007 ± 0.002, 3.6 ± 2.0a |
[2,8-3H]adenosine | 9.0 ± 2.0b | ||
[8-14C]2’-deoxyadenosine | 4.4 ± 0.8 | ||
adenosine | [2,8-3H]adenosine | 2.2 ± 1.0 | |
2 ’-deoxyadenosine | [8-14C]2’ -deoxyadenosine | 1.5 ± 0.1 | |
isopentenyl adenine | [8-14C]2’-deoxyadenosine | 0.0033 ± 0.0009 | |
trans-zeatin | [8-14C]2’-deoxyadenosine | 0.020 ± 0.003 | |
cis-zeatin | [8-14C]2’-deoxyadenosine | 0.190 ± 0.072 | |
PvENT4 | adenine | [2,8-3H]adenine | 0.006 ± 0.002, 3.2 ± 0.5a |
[2,8-3H]adenosine | 15.0 ± 0.5b | ||
[8-14C]2’-deoxyadenosine | 4.9 ± 2.3 | ||
adenosine | [2,8-3H]adenosine | 1.0 ± 0.2 | |
2 ’-deoxyadenosine | [8-14C]2’-deoxyadenosine | 4.3 ± 0.2 | |
isopentenyl adenine | [8-14C]2’-deoxyadenosine | 0.0095 ± 0.0028 | |
trans-zeatin | [8-14C]2’-deoxyadenosine | 0.193 ± 0.021 | |
cis-zeatin | [8-14C]2’-deoxyadenosine | 0.284 ± 0.069 |
These data were fit to a two-site competition model.
These data are the mean from two experiments, and the error is range/2.