One week of aspirin therapy improves responsiveness of leukocytes to certain TLR agonists. Fresh PBMCs were incubated overnight with TLR agonists CpG and HIV, and culture supernatants were stored at −20°C. Cytokine Bead Array analyses were performed on culture supernatants by flow cytometry. After 1 week of aspirin, more IL-6 was produced in response to CpGB, P = 0.01, more IL-6 was produced in response to HIV-1, P = 0.01, and more TNFα was produced in response to CpGB, P = 0.06 (all using paired t tests).