. Author manuscript; available in PMC: 2013 Aug 29.

Published in final edited form as: J Hered. 2007 Sep 1;98(6):555–566. doi: 10.1093/jhered/esm072

Table A2.

Development of a PCR-RFLP genotyping assay for (A) FGF5 mutations 1 and 2 and (B) Mutations 3 and 4 responsible for long hair in the domestic cat.

A
Mutation^b	Forward Primer	Reverse Primer	Restriction Enzyme	Undigested Product (bp)	Product Size (bp) following digestion^a
Mutation^b	Forward Primer	Reverse Primer	Restriction Enzyme	Undigested Product (bp)	Normal Allele	Mutant allele
Mutation 1	TGTAAAACGACGGCCAGTTCTACTGCAGAGTGGGCATC	GTGTCTTGCTTAGGACGCGCTTTGC	none	149	148	149
Mutation 2	GTGTCTTGTACCTCAGGGGATTGTAGGAGTA	TGTAAAACGACGGCCAGTTCTCCTGTAACACCTTTAACAAACA	RsaI	155	122	143

B
Mutation^b	Forward Primer	Reverse Primer	Restriction Enzyme	Undigested Product (bp)	Product Size (bp) following digestion^a
Mutation^b	Forward Primer	Reverse Primer	Restriction Enzyme	Undigested Product (bp)	No Mutation	Mutation 3	Mutation 4	Mutations 3 and 4
Mutation 3/Mutation 4	TGTAAAACGACGGCCAGTGACCTCATTTTATTAGATGCT	GTGTCTTACCGTCTCCCTGAACTTGCAGTCAACG	HpyCH4 III	132	98	97	122	121

The product sizes include the 1 bp non-templated A addition expected by the addition of the pig-tail sequence to one of the primers.

Mutations are coded as described in the text. 1 - c.ins356T, 2 - c.C>T406, 3 - c.del474T, 4 – c.A>C475 and 5 – no mutation.