Table A2.
A
| ||||||
---|---|---|---|---|---|---|
Mutationb | Forward Primer | Reverse Primer | Restriction Enzyme | Undigested Product (bp) | Product Size (bp) following digestiona
|
|
Normal Allele | Mutant allele | |||||
Mutation 1 | TGTAAAACGACGGCCAGTTCTACTGCAGAGTGGGCATC | GTGTCTTGCTTAGGACGCGCTTTGC | none | 149 | 148 | 149 |
Mutation 2 | GTGTCTTGTACCTCAGGGGATTGTAGGAGTA | TGTAAAACGACGGCCAGTTCTCCTGTAACACCTTTAACAAACA | RsaI | 155 | 122 | 143 |
B
| ||||||||
---|---|---|---|---|---|---|---|---|
Mutationb | Forward Primer | Reverse Primer | Restriction Enzyme | Undigested Product (bp) | Product Size (bp) following digestiona
|
|||
No Mutation | Mutation 3 | Mutation 4 | Mutations 3 and 4 | |||||
Mutation 3/Mutation 4 | TGTAAAACGACGGCCAGTGACCTCATTTTATTAGATGCT | GTGTCTTACCGTCTCCCTGAACTTGCAGTCAACG | HpyCH4 III | 132 | 98 | 97 | 122 | 121 |
The product sizes include the 1 bp non-templated A addition expected by the addition of the pig-tail sequence to one of the primers.
Mutations are coded as described in the text. 1 - c.ins356T, 2 - c.C>T406, 3 - c.del474T, 4 – c.A>C475 and 5 – no mutation.