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Detection of EGFP in MCF-7 breast cancer cells expressing EGFP, EGFP-LC3, or EGFP-ΔLC3 under non-starved conditions (a)-(c), and after autophagy induction by amino acid starvation for 3 hours (d)-(f). MCF-7 cells expressing EGFP (a) and (d), EGFP-LC3 (b) and (e), or EGFP-ΔLC3 (c) and (f) were imaged with confocal microscopy to show fluorescence intensity detected between 500 and 560 nm. These data are representative of at least 3 independent experiments.
