Figure 2.

Time course for AKT and ERK phosphorylation in EGF-stimulated rat goblet cells. Cultured rat conjunctival goblet cells were serum starved for 24 hours and then stimulated with EGF (10⁻⁷ M) for 0 to 10 minutes. Western blot analysis was performed using antibodies against phosphorylated and total AKT (A) and ERK (B). Representative blots from three experiments shown in figures on the left; data shown in the figures on the right represent mean ± SEM of three independent experiments. *Statistical significance compared with 0.