Figure 7.
Effect of inhibition of MEK, PI-3K, and PKC on constitutively active PKCα-stimulated cultured rat conjunctival goblet cell proliferation. Cultured rat conjunctival goblet cells were serum starved for 24 hours. The cells were preincubated with U0126 ([A], 10−7–10−5 M), LY294002 ([B], 10−7–10−5 M), or GÖ6983 ([C], 10−9–10−7 M) for 30 minutes prior to addition of an adenovirus containing myristolyated (active) PKCα (myrPKCα) (1 × 107 pfu) or with no addition for 22 hours. The number of proliferating cells was determined by WST-8. Data are mean ± SEM from three independent experiments. Dotted line indicates basal value, set to 1.0. *Statistically significant difference from basal. #Statistically significant difference from myrPKCα.