Figure 9.
Effect of inhibition of Src on EGF-stimulated activation of Src, AKT, and ERK in rat conjunctival goblet cells. Cultured rat conjunctival goblet cells were serum starved for 24 hours and preincubated with PP1 (10−6–10−4 M) for 30 minutes prior to addition of EGF for 5 minutes. Western blot analysis was performed using antibodies against phosphorylated and total Src (A, B), phosphorylated and total AKT (A, C), and phosphorylated ERK and total ERK (A, D). The blots shown in (A) are representative of three independent experiments. The blots were scanned, and the data shown in (B–D) represent mean ± SEM of three experiments. *Statistical significance compared with basal, set to 1.0. #Statistical significance compared with EGF alone.