. Author manuscript; available in PMC: 2014 Feb 1.

Published in final edited form as: Proteomics. 2012 Nov 26;13(0):674–685. doi: 10.1002/pmic.201200271

Table 4.

The molecular weight and isoelectric point of A2M (35 kDa subunit) isoforms

Isoform #	Theoretical MW (kDa) ^a	Observed MW (kDa) ^b	Theoretical pI ^a	Observed pI ^b	Theoretical pI with possible phosphorylation (P) ^c
1	28.2	38.3	7.09	5.65	5.57 (4P)
2	28.2	38.7	7.09	5.85	5.81 (3P)
3	28.2	38.5	7.09	6.08	6.08 (2P)
4	28.2	38	7.09	6.3	6.43 (1P)
5	28.2	37.7	7.09	6.54
6	28.2	37.5	7.09	6.80
7	28.2	37.6	7.09	7.08	7.09 (0P)

MW and pI were calculated at http://scansite.mit.edu/calc_mw_pi.html.

Observed MW and pI were estimated from the 2-D gel

Theoretical pIs assuming phosphorylation were calculated at http://scansite.mit.edu/calc_mw_pi.html. 1P means one phosphorylation and 2P means two phosphorylations, etc.