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Mutations of phosphorylation sites in β-catenin would not affect the transcription activation by NLK-S.
The dual luciferase assays of pTOPFLASH with stabilized β-catenin in SaOS-2 cells were shown. The amount of MYC-β-catenin ΔN mutants and NLK-S expression vectors used was 0.1 μg/well.
