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. 2013 Aug 28;33(35):13978–13988. doi: 10.1523/JNEUROSCI.2383-13.2013

Figure 3.

Figure 3.

Social defeat decreased excitability and enhanced inhibitory input of DRN 5-HT neurons of SUS mice. A, Raw data traces of membrane potential changes recorded from DRN 5-HT neurons in response to hyperpolarizing and depolarizing current injection in control, RES, and SUS mice. Scale bar, 20 mV, 50 ms. B, Representative traces of mIPSC activity in DRN 5-HT neurons from control, RES, and SUS mice. Scale bar, 20 pA, 2 s. C, Schematic of DRN topography in recorded slices from Pet1-tdTomato mice. dm/vmDR, dorsomedial/ventromedial DRN; Aq, aqueduct. D, Frequency-intensity plots show decreased excitability of DRN 5-HT neurons in SUS mice [repeated-measures ANOVA, Fisher's post hoc; SUS vs CTRL, 60 pA *p = 0.043, 80 pA *p = 0.024, 100 pA *p = 0.023, 120 pA *p = 0.033; N(n) = 3(15) for control, 3(16) for RES, 3(21) for SUS]. E, Cumulative probability plots show the shift to shorter interstimulus intervals of mIPSC activity in DRN 5-HT neurons of SUS mice versus control or RES mice (Kolmogorov–Smirnov test, Z = 8.652, p < 0.001). Inset displays summary histogram depicting the significantly higher average mIPSC frequency in SUS mice (one-way ANOVA, Fisher's post hoc; SUS vs CTRL *p = 0.035).