Figure 3.

The gross morphology of the inner ear is unaltered in Vangl2 CKOs. Medial views of the 3D reconstructions of control (A) and Vangl2 CKO (B) inner ears at E18.5 show similar size and morphology of the endolymphatic spaces, including the length and spiral coil of the cochlea. Auditory (blue) and vestibular (red) structures were highlighted by pseudocoloring after reconstruction. C, The inner ear from Vangl2 KOs shows a reduction in size dorsoventrally and a shortened cochlear duct. Ventral views of control (D) and Vangl2 CKO (E) scala media demonstrate the similar length and number of turns of the cochlea in these genotypes. The ventral view of Vangl2 KO scala media (F) shows a reduction in the length and number of turns to the cochlea duct. Scale bars, 200 μm.